Ontology highlight
ABSTRACT:
SUBMITTER: Vodak D
PROVIDER: S-EPMC5871786 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Vodák Daniel D Lorenz Susanne S Nakken Sigve S Aasheim Lars Birger LB Holte Harald H Bai Baoyan B Myklebost Ola O Meza-Zepeda Leonardo A LA Hovig Eivind E
Scientific reports 20180328 1
Sample pooling enabled by dedicated indexes is a common strategy for cost-effective and robust high-throughput sequencing. Index misassignment leading to mutual contamination between pooled samples has however been described as a general problem of the latest Illumina sequencing instruments utilizing exclusion amplification. Using real-life data from multiple tumour sequencing projects, we demonstrate that index misassignment can induce artefactual variant calls closely resembling true, high-qua ...[more]