A Molecular Method for the Identification of Honey Bee Subspecies Used by Beekeepers in Russia.

ABSTRACT: Apis mellifera L. includes several recognized subspecies that differ in their biological properties and agricultural characteristics. Distinguishing between honey bee subspecies is complicated. We analyzed the Folmer region of the COX1 gene in honey bee subspecies cultivated at bee farms in Russia and identified subspecies-specific SNPs. DNA analysis revealed two clearly distinct haplogroups in A. melliferamellifera. The first one was characterized by multiple cytosine-thymine (thymine-cytosine) transitions, one adenine-guanine substitution, and one thymine-adenine substitution. The nucleotide sequence of the second haplogroup coincided with sequences from other subspecies, except the unique C/A SNP at position 421 of the 658-bp Folmer region. A. mellifera

SUBMITTER: Syromyatnikov MY 

PROVIDER: S-EPMC5872275 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature