Ontology highlight
ABSTRACT:
SUBMITTER: Harrington LB
PROVIDER: S-EPMC5875921 | biostudies-literature | 2017 Sep
REPOSITORIES: biostudies-literature
Harrington Lucas B LB Doxzen Kevin W KW Ma Enbo E Liu Jun-Jie JJ Knott Gavin J GJ Edraki Alireza A Garcia Bianca B Amrani Nadia N Chen Janice S JS Cofsky Joshua C JC Kranzusch Philip J PJ Sontheimer Erik J EJ Davidson Alan R AR Maxwell Karen L KL Doudna Jennifer A JA
Cell 20170824 6
CRISPR-Cas9 proteins function within bacterial immune systems to target and destroy invasive DNA and have been harnessed as a robust technology for genome editing. Small bacteriophage-encoded anti-CRISPR proteins (Acrs) can inactivate Cas9, providing an efficient off switch for Cas9-based applications. Here, we show that two Acrs, AcrIIC1 and AcrIIC3, inhibit Cas9 by distinct strategies. AcrIIC1 is a broad-spectrum Cas9 inhibitor that prevents DNA cutting by multiple divergent Cas9 orthologs thr ...[more]