Ontology highlight
ABSTRACT:
SUBMITTER: Scalise M
PROVIDER: S-EPMC5877509 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Scalise Mariafrancesca M Pochini Lorena L Console Lara L Pappacoda Gilda G Pingitore Piero P Hedfalk Kristina K Indiveri Cesare C
International journal of molecular sciences 20180225 3
The human plasma membrane transporter ASCT2 is responsible for mediating Na- dependent antiport of neutral amino acids. New insights into structure/function relationships were unveiled by a combined approach of recombinant over-expression, site-directed mutagenesis, transport assays in proteoliposomes and bioinformatics. WT and Cys mutants of hASCT2 were produced in <i>P. pastoris</i> and purified for functional assay. The reactivity towards SH reducing and oxidizing agents of WT protein was inv ...[more]