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The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation.

ABSTRACT: The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish adamts5 during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of myod expression by ADAMTS5 and Shh during somite differentiation was observed. These roles were not dependent upon the catalytic activity of ADAMTS5. These data identify a non-enzymatic function for ADAMTS5 in regulating an important cell signaling pathway that impacts on muscle development, with implications for musculoskeletal diseases in which ADAMTS5 and Shh have been associated.

SUBMITTER: Dancevic CM

PROVIDER: S-EPMC5877627 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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The ADAMTS5 Metzincin Regulates Zebrafish Somite Differentiation.

Dancevic Carolyn M CM Gibert Yann Y Berger Joachim J Smith Adam D AD Liongue Clifford C Stupka Nicole N Ward Alister C AC McCulloch Daniel R DR

International journal of molecular sciences 20180307 3

The ADAMTS5 metzincin, a secreted zinc-dependent metalloproteinase, modulates the extracellular matrix (ECM) during limb morphogenesis and other developmental processes. Here, the role of ADAMTS5 was investigated by knockdown of zebrafish <i>adamts5</i> during embryogenesis. This revealed impaired Sonic Hedgehog (Shh) signaling during somite patterning and early myogenesis. Notably, synergistic regulation of <i>myod</i> expression by ADAMTS5 and Shh during somite differentiation was observed. Th ...[more]

PMID: 29518972

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