Single-molecule peptide fingerprinting.
Ontology highlight
ABSTRACT:
SUBMITTER: van Ginkel J
PROVIDER: S-EPMC5879649 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Publications
Single-molecule peptide fingerprinting.
van Ginkel Jetty J Filius Mike M Szczepaniak Malwina M Tulinski Pawel P Meyer Anne S AS Joo Chirlmin C
Proceedings of the National Academy of Sciences of the United States of America 20180312 13
Proteomic analyses provide essential information on molecular pathways of cellular systems and the state of a living organism. Mass spectrometry is currently the first choice for proteomic analysis. However, the requirement for a large amount of sample renders a small-scale proteomics study challenging. Here, we demonstrate a proof of concept of single-molecule FRET-based protein fingerprinting. We harnessed the AAA+ protease ClpXP to scan peptides. By using donor fluorophore-labeled ClpP, we se ...[more]
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