Ontology highlight
ABSTRACT:
SUBMITTER: Rossi MJ
PROVIDER: S-EPMC5880240 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Genome research 20180321 4
General regulatory factors (GRFs), such as Reb1, Abf1, Rap1, Mcm1, and Cbf1, positionally organize yeast chromatin through interactions with a core consensus DNA sequence. It is assumed that sequence recognition via direct base readout suffices for specificity and that spurious nonfunctional sites are rendered inaccessible by chromatin. We tested these assumptions through genome-wide mapping of GRFs in vivo and in purified biochemical systems at near-base pair (bp) resolution using several ChIP- ...[more]