Immobilization alters heparin cleaving properties of heparinase I.
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ABSTRACT:
SUBMITTER: Bhushan I
PROVIDER: S-EPMC5881678 | biostudies-literature | 2017 Nov
REPOSITORIES: biostudies-literature
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Immobilization alters heparin cleaving properties of heparinase I.
Bhushan Indu I Alabbas Alhumaidi A Kuberan Balagurunathan B Gupta Ram B RB Desai Umesh R UR
Glycobiology 20171101 11
We report here a novel observation that immobilization of heparinase I on CNBr-activated Sepharose results in heparin degradation properties that are different from heparinase I in the free solution form. Studies over a range of pHs (5-8) and temperatures (5-50°C) as well as under batch and flow conditions show that immobilized heparinase 1 displays altered pH and temperature optima, and a higher propensity for generation of longer chains (hexa- and octa-) with variable sulfation as compared to ...[more]
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