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New endoperoxides highly active in vivo and in vitro against artemisinin-resistant Plasmodium falciparum.


ABSTRACT: BACKGROUND:The emergence and spread of Plasmodium falciparum resistance to artemisinin-based combination therapy in Southeast Asia prompted the need to develop new endoperoxide-type drugs. METHODS:A chemically diverse library of endoperoxides was designed and synthesized. The compounds were screened for in vitro and in vivo anti-malarial activity using, respectively, the SYBR Green I assay and a mouse model. Ring survival and mature stage survival assays were performed against artemisinin-resistant and artemisinin-sensitive P. falciparum strains. Cytotoxicity was evaluated against mammalian cell lines V79 and HepG2, using the MTT assay. RESULTS:The synthesis and anti-malarial activity of 21 new endoperoxide-derived compounds is reported, where the peroxide pharmacophore is part of a trioxolane (ozonide) or a tetraoxane moiety, flanked by adamantane and a substituted cyclohexyl ring. Eight compounds exhibited sub-micromolar anti-malarial activity (IC50 0.3-71.1 nM), no cross-resistance with artemisinin or quinolone derivatives and negligible cytotoxicity towards mammalian cells. From these, six produced ring stage survival

SUBMITTER: Lobo L 

PROVIDER: S-EPMC5883364 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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New endoperoxides highly active in vivo and in vitro against artemisinin-resistant Plasmodium falciparum.

Lobo Lis L   Cabral Lília I L LIL   Sena Maria Inês MI   Guerreiro Bruno B   Rodrigues António Sebastião AS   de Andrade-Neto Valter Ferreira VF   Cristiano Maria L S MLS   Nogueira Fatima F  

Malaria journal 20180403 1


<h4>Background</h4>The emergence and spread of Plasmodium falciparum resistance to artemisinin-based combination therapy in Southeast Asia prompted the need to develop new endoperoxide-type drugs.<h4>Methods</h4>A chemically diverse library of endoperoxides was designed and synthesized. The compounds were screened for in vitro and in vivo anti-malarial activity using, respectively, the SYBR Green I assay and a mouse model. Ring survival and mature stage survival assays were performed against art  ...[more]

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