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Enhanced Ordering in Monolayers Containing Glycosphingolipids: Impact of Carbohydrate Structure.


ABSTRACT: The influence of carbohydrate structure on the ordering of glycosphingolipids (GSLs) and surrounding phospholipids was investigated in monolayers at the air-water interface. Binary mixtures composed of GSLs, chosen to span a range of carbohydrate complexity, and zwitterionic dipalmitoylphosphatidylcholine phospholipid, were studied. X-ray reflectivity was used to measure the out-of-plane structure of the monolayers and characterize the extension and conformation of the GSL carbohydrates. Using synchrotron grazing incidence x-ray diffraction, the in-plane packing of the lipid acyl chains and the area per molecule within ordered domains were characterized at different mole ratios of the two components. Our findings indicate that GSL-containing mixtures, regardless of the carbohydrate size, enhance the ordering of the surrounding lipids, resulting in a larger fraction of ordered phase of the monolayer and greater dimensions of the ordered domains. Reduction of the averaged area per molecule within the ordered domains was also observed but only in the cases where there was a size mismatch between the phospholipid headgroups and GSL components, suggesting that the condensation mechanism involves the relief of steric interactions between headgroups in mixtures.

SUBMITTER: Watkins EB 

PROVIDER: S-EPMC5883550 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Enhanced Ordering in Monolayers Containing Glycosphingolipids: Impact of Carbohydrate Structure.

Watkins Erik B EB   Frey Shelli L SL   Chi Eva Y EY   Cao Kathleen D KD   Pacuszka Tadeusz T   Majewski Jaroslaw J   Lee Ka Yee C KYC  

Biophysical journal 20180301 5


The influence of carbohydrate structure on the ordering of glycosphingolipids (GSLs) and surrounding phospholipids was investigated in monolayers at the air-water interface. Binary mixtures composed of GSLs, chosen to span a range of carbohydrate complexity, and zwitterionic dipalmitoylphosphatidylcholine phospholipid, were studied. X-ray reflectivity was used to measure the out-of-plane structure of the monolayers and characterize the extension and conformation of the GSL carbohydrates. Using s  ...[more]

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