Thy1-Targeted Microbubbles for Ultrasound Molecular Imaging of Pancreatic Ductal Adenocarcinoma.

ABSTRACT: Purpose: To engineer a dual human and murine Thy1-binding single-chain-antibody ligand (Thy1-scFv) for contrast microbubble-enhanced ultrasound molecular imaging of pancreatic ductal adenocarcinoma (PDAC).Experimental Design: Thy1-scFv were engineered using yeast-surface-display techniques. Binding to soluble human and murine Thy1 and to Thy1-expressing cells was assessed by flow cytometry. Thy1-scFv was then attached to gas-filled microbubbles to create MB_Thy1-scFv Thy1 binding of MB_Thy1-scFv to Thy1-expressing cells was evaluated under flow shear stress conditions in flow-chamber experiments. MB_{scFv-scrambled} and MB_Non-targeted were used as negative controls. All microbubble types were tested in both orthotopic human PDAC xenograf

SUBMITTER: Abou-Elkacem L 

PROVIDER: S-EPMC5884723 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature