Unknown

Dataset Information

0

Tissue microarray is suitable for scientific biomarkers studies in endometrial cancer.


ABSTRACT: The aim of this study was to define the concordance between tissue microarrays (TMAs) of different sizes and whole slide for 15 different antibodies in endometrial cancer and study the use of TMAs in preoperative endometrial samples. Cores of preoperative and hysterectomy specimens of 14 endometrial cancer and three atypical hyperplasia cases were collected in TMA blocks. Two 0.6-mm and two 2.0-mm cores were used from each sample. Different antibodies were tested in TMAs and compared with results of whole slides of hysterectomy. Tested antibodies were as follows: ER, PR, p53, Ki-67, MLH1, PMS2, MSH2, MSH6, ARID1A, stathmin, IMP3, L1CAM, PTEN, ?-catenin, and p16. Seventeen cases with four cores per paraffin block (both 0.6 and 2.0 mm in duplicate) and 15 different antibodies resulted in a total of 1020 cores for both preoperative and hysterectomy specimen. Overall, 2.0-mm cores were more assessable for evaluation than 0.6-mm cores (96.0 versus 79.5%, p?

SUBMITTER: Visser NCM 

PROVIDER: S-EPMC5887002 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Tissue microarray is suitable for scientific biomarkers studies in endometrial cancer.

Visser Nicole C M NCM   van der Wurff Anneke A M AAM   Pijnenborg Johanna M A JMA   Massuger Leon F A G LFAG   Bulten Johan J   Nagtegaal Iris D ID  

Virchows Archiv : an international journal of pathology 20180209 3


The aim of this study was to define the concordance between tissue microarrays (TMAs) of different sizes and whole slide for 15 different antibodies in endometrial cancer and study the use of TMAs in preoperative endometrial samples. Cores of preoperative and hysterectomy specimens of 14 endometrial cancer and three atypical hyperplasia cases were collected in TMA blocks. Two 0.6-mm and two 2.0-mm cores were used from each sample. Different antibodies were tested in TMAs and compared with result  ...[more]

Similar Datasets

| S-EPMC5342519 | biostudies-literature
| S-EPMC7008300 | biostudies-literature
| S-EPMC1184647 | biostudies-other
| S-EPMC6519061 | biostudies-literature
| S-EPMC4490552 | biostudies-literature
| S-EPMC5787511 | biostudies-other
| S-EPMC2561043 | biostudies-literature
| S-EPMC8315102 | biostudies-literature
| S-EPMC7428878 | biostudies-literature
| S-SCDT-10_1038-S44321-024-00033-1 | biostudies-other