Ontology highlight
ABSTRACT:
SUBMITTER: Bell CC
PROVIDER: S-EPMC5888952 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Bell Catherine C CC Dankers Anita C A ACA Lauschke Volker M VM Sison-Young Rowena R Jenkins Roz R Rowe Cliff C Goldring Chris E CE Park Kevin K Regan Sophie L SL Walker Tracy T Schofield Chris C Baze Audrey A Foster Alison J AJ Williams Dominic P DP van de Ven Amy W M AWM Jacobs Frank F Houdt Jos van JV Lähteenmäki Tuula T Snoeys Jan J Juhila Satu S Richert Lysiane L Ingelman-Sundberg Magnus M
Toxicological sciences : an official journal of the Society of Toxicology 20180401 2
Primary human hepatocytes (PHHs) are commonly used for in vitro studies of drug-induced liver injury. However, when cultured as 2D monolayers, PHH lose crucial hepatic functions within hours. This dedifferentiation can be ameliorated when PHHs are cultured in sandwich configuration (2Dsw), particularly when cultures are regularly re-overlaid with extracellular matrix, or as 3D spheroids. In this study, the 6 participating laboratories evaluated the robustness of these 2 model systems made from c ...[more]