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Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin.


ABSTRACT: Objectives:We evaluate the performance characteristics of antiphosphatidylserine (anti-PS), antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti-?2 glycoprotein I (anti-?2GPI) in a large group of patients with antiphospholipid (aPL)-related diseases. Methods:Serum samples from 548 patients from the Hopkins and Jamaican systemic lupus erythematosus cohorts, the PROMISSE cohort, and the Antiphospholipid Standardization Laboratory were examined for immunoglobulin G (IgG)/immunoglobulin M (IgM) positivity in aCL, anti-?2GPI, anti-PS, anti-PI, and APhL ELISA assays. Results:All IgG assays were associated with one or more thrombotic and/or obstetric manifestations, with an increased risk associated with higher antibody titers. Analytical performance was similar among assays, but IgG assays performed better than IgM counterparts. Conclusions:Increasing titers of APhL, anti-PS, and anti-PI antibodies could indicate an increased risk of thrombotic and/or obstetric aPL-related manifestations. These assays may be promising biomarkers for particular APS manifestations.

SUBMITTER: Castanon A 

PROVIDER: S-EPMC5889035 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin.

Castanon Amaris A   Pierre Grant G   Willis Rohan R   Harris E Nigel EN   Papalardo Elizabeth E   Romay-Penabad Zurina Z   Schleh Alvaro A   Jajoria Praveen P   Smikle Monica M   DeCeulaer Karel K   Tebo Anne A   Jaskowski Troy T   Guerra Marta M MM   Branch D Ware DW   Salmon Jane E JE   Petri Michelle M   Gonzalez Emilio B EB  

American journal of clinical pathology 20180301 5


<h4>Objectives</h4>We evaluate the performance characteristics of antiphosphatidylserine (anti-PS), antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti-β2 glycoprotein I (anti-β2GPI) in a large group of patients with antiphospholipid (aPL)-related diseases.<h4>Methods</h4>Serum samples from 548 patients from the Hopkins and Jamaican systemic lupus erythematosus cohorts, the PROMISSE cohor  ...[more]

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