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Multiple system atrophy and apolipoprotein E.

ABSTRACT: BACKGROUND:Dysregulation of the specialized lipid metabolism involved in myelin synthesis and maintenance by oligodendrocytes has been associated with the unique neuropathology of MSA. We hypothesized that apolipoprotein E, which is associated with neurodegeneration, may also play a role in the pathogenesis of MSA. OBJECTIVE:This study evaluated genetic associations of Apolipoprotein E alleles with risk of MSA and ?-synuclein pathology, and also examined whether apolipoprotein E isoforms differentially affect ?-synuclein uptake in a oligodendrocyte cell. METHODS:One hundred sixty-eight pathologically confirmed MSA patients, 89 clinically diagnosed MSA patients, and 1,277 control subjects were genotyped for Apolipoprotein E. Human oligodendrocyte cell lines were incubated with ?-synuclein and recombinant human apolipoprotein E, with internalized ?-synuclein imaged by confocal microscopy and cells analyzed by flow cytometry. RESULTS:No significant association with risk of MSA or was observed for either Apolipoprotein E ?2 or ?4. ?-Synuclein burden was also not associated with Apolipoprotein E alleles in the pathologically confirmed patients. Interestingly, in our cell assays, apolipoprotein E ?4 significantly reduced ?-synuclein uptake in the oligodendrocytic cell line. CONCLUSIONS:Despite differential effects of apolipoprotein E isoforms on ?-synuclein uptake in a human oligodendrocytic cell, we did not observe a significant association at the Apolipoprotein E locus with risk of MSA or ?-synuclein pathology. © 2018 International Parkinson and Movement Disorder Society.

SUBMITTER: Ogaki K

PROVIDER: S-EPMC5889322 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Multiple system atrophy and apolipoprotein E.

Ogaki Kotaro K Martens Yuka A YA Heckman Michael G MG Koga Shunsuke S Labbé Catherine C Lorenzo-Betancor Oswaldo O Wernick Anna I AI Walton Ronald L RL Soto Alexandra I AI Vargas Emily R ER Nielsen Henrietta M HM Fujioka Shinsuke S Kanekiyo Takahisa T Uitti Ryan J RJ van Gerpen Jay A JA Cheshire William P WP Wszolek Zbigniew K ZK Low Phillip A PA Singer Wolfgang W Dickson Dennis W DW Bu Guojun G Ross Owen A OA

Movement disorders : official journal of the Movement Disorder Society 20180214 4

<h4>Background</h4>Dysregulation of the specialized lipid metabolism involved in myelin synthesis and maintenance by oligodendrocytes has been associated with the unique neuropathology of MSA. We hypothesized that apolipoprotein E, which is associated with neurodegeneration, may also play a role in the pathogenesis of MSA.<h4>Objective</h4>This study evaluated genetic associations of Apolipoprotein E alleles with risk of MSA and α-synuclein pathology, and also examined whether apolipoprotein E i ...[more]

PMID: 29442376

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Project description:Multiple system atrophy (MSA) is an orphan, fatal, adult-onset neurodegenerative disorder of uncertain etiology that is clinically characterized by various combinations of parkinsonism, cerebellar, autonomic, and motor dysfunction. MSA is an ?-synucleinopathy with specific glioneuronal degeneration involving striatonigral, olivopontocerebellar, and autonomic nervous systems but also other parts of the central and peripheral nervous systems. The major clinical variants correlate with the morphologic phenotypes of striatonigral degeneration (MSA-P) and olivopontocerebellar atrophy (MSA-C). While our knowledge of the molecular pathogenesis of this devastating disease is still incomplete, updated consensus criteria and combined fluid and imaging biomarkers have increased its diagnostic accuracy. The neuropathologic hallmark of this unique proteinopathy is the deposition of aberrant ?-synuclein in both glia (mainly oligodendroglia) and neurons forming glial and neuronal cytoplasmic inclusions that cause cell dysfunction and demise. In addition, there is widespread demyelination, the pathogenesis of which is not fully understood. The pathogenesis of MSA is characterized by propagation of misfolded ?-synuclein from neurons to oligodendroglia and cell-to-cell spreading in a "prion-like" manner, oxidative stress, proteasomal and mitochondrial dysfunction, dysregulation of myelin lipids, decreased neurotrophic factors, neuroinflammation, and energy failure. The combination of these mechanisms finally results in a system-specific pattern of neurodegeneration and a multisystem involvement that are specific for MSA. Despite several pharmacological approaches in MSA models, addressing these pathogenic mechanisms, no effective neuroprotective nor disease-modifying therapeutic strategies are currently available. Multidisciplinary research to elucidate the genetic and molecular background of the deleterious cycle of noxious processes, to develop reliable biomarkers and targets for effective treatment of this hitherto incurable disorder is urgently needed.

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Multiple system atrophy and apolipoprotein E.

Publications

Multiple system atrophy and apolipoprotein E.

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