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ABSTRACT: Objectives
There is an urgent need for accurate and fast diagnostic tests capable of identifying carbapenemase producers. Here, we assessed the performance of a new multiplex lateral flow assay (OKN K -SeT) for the rapid detection of OXA-48-like, KPC and NDM carbapenemase-producing Enterobacteriaceae from culture colonies.Methods
Two hundred collection isolates with characterized ?-lactamase content and 183 non-duplicate consecutive isolates referred to two National Reference Centres over a 2?month period in 2016 were used to evaluate the OKN K -SeT assay.Results
The assay correctly detected all 42 OXA-48-like-, 27 KPC- and 30 NDM-producing isolates from the collection panel, including 7 isolates that co-produced NDM and OXA-181 carbapenemases. No cross-reactivity was observed with non-targeted carbapenemases ( n? = ? 41) or with non-carbapenemase producers ( n? = ? 60). Prospectively, all OXA-48-like ( n? = ? 69), KPC ( n? = ? 9) and NDM ( n? = ? 19) carbapenemase-producing Enterobacteriaceae isolates were correctly detected, while 11 carbapenemase producers not targeted by the assay went undetected [VIM ( n? = ? 8) and OXA-23/OXA-58-like ( n? = ? 3)]. Overall, the sensitivity and specificity of the assay were 100%.Conclusions
The OKN assay is efficient, rapid and easy to implement in the workflow of a clinical microbiology laboratory for the confirmation of OXA-48, NDM and KPC carbapenemases. This test represents a powerful diagnostic tool as it enables the rapid detection of the most clinically important carbapenemases without the need for more costly and less frequently available molecular assays.
SUBMITTER: Glupczynski Y
PROVIDER: S-EPMC5890672 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Glupczynski Youri Y Jousset Agnès A Evrard Stéphanie S Bonnin Rémy A RA Huang Te-Din TD Dortet Laurent L Bogaerts Pierre P Naas Thierry T
The Journal of antimicrobial chemotherapy 20170701 7
<h4>Objectives</h4>There is an urgent need for accurate and fast diagnostic tests capable of identifying carbapenemase producers. Here, we assessed the performance of a new multiplex lateral flow assay (OKN K -SeT) for the rapid detection of OXA-48-like, KPC and NDM carbapenemase-producing Enterobacteriaceae from culture colonies.<h4>Methods</h4>Two hundred collection isolates with characterized β-lactamase content and 183 non-duplicate consecutive isolates referred to two National Reference Cen ...[more]