Ontology highlight
ABSTRACT:
SUBMITTER: Li XL
PROVIDER: S-EPMC5890932 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Li Xin-Liang XL Ljungdahl Lars G LG Ximenes Eduardo A EA Chen Huizhong H Felix Carlos R CR Cotta Michael A MA Dien Bruce S BS
Applied biochemistry and biotechnology 20040101
A beta-glucosidase (BglA, EC 3.2.1.21) gene from the polycentric anaerobic fungus Orpinomyces PC-2 was cloned and sequenced. The enzyme containing 657 amino acid residues was homologous to certain animal, plant, and bacterial beta-glucosidases but lacked significant similarity to those from aerobic fungi. Neither cellulose- nor protein-binding domains were found in BglA. When expressed in Saccharomyces cerevisiae, the enzyme was secreted in two forms with masses of about 110 kDa and also found i ...[more]