Ontology highlight
ABSTRACT:
SUBMITTER: Kane SD
PROVIDER: S-EPMC5892457 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Enzyme and microbial technology 20180215
Recent analyses of genome sequences belonging to cellulolytic bacteria have revealed many genes potentially coding for cellulosic biomass degradation enzymes. Annotation of these genes however, is based on few biochemically characterised examples. Here we present a simple strategy based on BioBricks for the rapid screening of candidate genes expressed in Escherichia coli. As proof of principle we identified over 70 putative biomass degrading genes from bacterium Cellulomonas fimi, expressing a s ...[more]