Dataset Information

Distinct In Vitro T-Helper 17 Differentiation Capacity of Peripheral Naive T Cells in Rheumatoid and Psoriatic Arthritis.

ABSTRACT:

Background

The T-helper 17 (Th17) cells have a prominent role in inflammation as well as in bone and join destruction in both rheumatoid and psoriatic arthritis (RA and PsA). Here, we studied Th17?cell differentiation in RA and PsA.

Methods

Blood samples from healthy donors, RA and PsA patients were collected. CD45RO^- (naive) and CD45RO⁺ (memory) T cells were isolated from peripherial blood mononuclear cell by magnetic separation. Naive T cells were stimulated with anti-CD3, anti-CD28, and goat anti-mouse IgG antibodies and treated with transforming grow factor beta, interleukin (IL)-6, IL-1_?, and IL-23 cytokines and also with anti-IL-4 antibody. IL-17A and IL-22 production were measured by enzyme linked immunosorbent assay, RORC, and T-box 21 (TBX21) expression were analyzed by quantitative polymerase chain reaction and flow cytometry. C-C chemokine receptor 6 (CCR6), CCR4, and C-X-C motif chemokine receptor 3 expression were determined by flow cytometry. Cell viability was monitored by impedance-based cell analyzer (CASY-TT).

Results

RORC, TBX21, CCR6, and CCR4 expression of memory T cells of healthy individuals (but not RA or PsA patients) were increased (p?p?p?p?p?=?0.0000026 and p?=?0.0001047, respectively). By contrast, significant differences in IL-22 production were observed only between RA versus healthy or RA versus PsA patients (p?=?0.000006; p?=?0.0013454, respectively), but not between healthy donors versus PsA patients.

Conclusion

The naive CD4 T-lymphocytes are predisposed to differentiate into Th17?cells and the in vitro Th17 cell differentiation is profoundly altered in both RA and PsA.

SUBMITTER: Baricza E

PROVIDER: S-EPMC5893718 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Distinct <i>In Vitro</i> T-Helper 17 Differentiation Capacity of Peripheral Naive T Cells in Rheumatoid and Psoriatic Arthritis.

Baricza Eszter E Marton Nikolett N Királyhidi Panna P Kovács Orsolya Tünde OT Kovácsné Székely Ilona I Lajkó Eszter E Kőhidai Lászó L Rojkovich Bernadett B Érsek Barbara B Buzás Edit Irén EI Nagy György G

Frontiers in immunology 20180404

<h4>Background</h4>The T-helper 17 (Th17) cells have a prominent role in inflammation as well as in bone and join destruction in both rheumatoid and psoriatic arthritis (RA and PsA). Here, we studied Th17 cell differentiation in RA and PsA.<h4>Methods</h4>Blood samples from healthy donors, RA and PsA patients were collected. CD45RO<sup>-</sup> (naive) and CD45RO<sup>+</sup> (memory) T cells were isolated from peripherial blood mononuclear cell by magnetic separation. Naive T cells were stimulate ...[more]

PMID: 29670615

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