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Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production.


ABSTRACT: Sustainable and eco-efficient alternatives for the production of platform chemicals, fuels and chemical building blocks require the development of stable, reusable and recyclable biocatalysts. Here we present a novel concept for the biocatalytic production of 1,5-diaminopentane (DAP, trivial name: cadaverine) using catalytically active inclusion bodies (CatIBs) of the constitutive L-lysine decarboxylase from E. coli (EcLDCc-CatIBs) to process L-lysine-containing culture supernatants from Corynebacterium glutamicum. EcLDCc-CatIBs can easily be produced in E. coli followed by a simple purification protocol yielding up to 43% dry CatIBs per dry cell weight. The stability and recyclability of EcLDCc-CatIBs was demonstrated in (repetitive) batch experiments starting from L-lysine concentrations of 0.1?M and 1?M. EcLDC-CatIBs exhibited great stability under reaction conditions with an estimated half-life of about 54?h. High conversions to DAP of 87-100% were obtained in 30-60?ml batch reactions using approx. 180-300?mg EcLDCc-CatIBs, respectively. This resulted in DAP titres of up to 88.4?g?l-1 and space-time yields of up to 660 gDAP l-1 d-1 per gram dry EcLDCc-CatIBs. The new process for DAP production can therefore compete with the currently best fermentative process as described in the literature.

SUBMITTER: Kloss R 

PROVIDER: S-EPMC5895699 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production.

Kloss Ramona R   Limberg Michael H MH   Mackfeld Ursula U   Hahn Doris D   Grünberger Alexander A   Jäger Vera D VD   Krauss Ulrich U   Oldiges Marco M   Pohl Martina M  

Scientific reports 20180411 1


Sustainable and eco-efficient alternatives for the production of platform chemicals, fuels and chemical building blocks require the development of stable, reusable and recyclable biocatalysts. Here we present a novel concept for the biocatalytic production of 1,5-diaminopentane (DAP, trivial name: cadaverine) using catalytically active inclusion bodies (CatIBs) of the constitutive L-lysine decarboxylase from E. coli (EcLDCc-CatIBs) to process L-lysine-containing culture supernatants from Coryneb  ...[more]

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