Codon usage bias controls mRNA and protein abundance in trypanosomatids.
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ABSTRACT:
SUBMITTER: Jeacock L
PROVIDER: S-EPMC5896881 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Publications
Codon usage bias controls mRNA and protein abundance in trypanosomatids.
Jeacock Laura L Faria Joana J Horn David D
eLife 20180315
Protein abundance differs from a few to millions of copies per cell. <i>Trypanosoma brucei</i> presents an excellent model for studies on codon bias and differential gene expression because transcription is broadly unregulated and uniform across the genome. <i>T. brucei</i> is also a major human and animal protozoal pathogen. Here, an experimental assessment, using synthetic reporter genes, revealed that GC3 codons have a major positive impact on both mRNA and protein abundance. Our estimates of ...[more]
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