Unknown

Dataset Information

0

ICE1 promotes the link between splicing and nonsense-mediated mRNA decay.


ABSTRACT: The nonsense-mediated mRNA decay (NMD) pathway detects aberrant transcripts containing premature termination codons (PTCs) and regulates expression of 5-10% of non-aberrant human mRNAs. To date, most proteins involved in NMD have been identified by genetic screens in model organisms; however, the increased complexity of gene expression regulation in human cells suggests that additional proteins may participate in the human NMD pathway. To identify proteins required for NMD, we performed a genome-wide RNAi screen against >21,000 genes. Canonical members of the NMD pathway were highly enriched as top hits in the siRNA screen, along with numerous candidate NMD factors, including the conserved ICE1/KIAA0947 protein. RNAseq studies reveal that depletion of ICE1 globally enhances accumulation and stability of NMD-target mRNAs. Further, our data suggest that ICE1 uses a putative MIF4G domain to interact with exon junction complex (EJC) proteins and promotes the association of the NMD protein UPF3B with the EJC.

SUBMITTER: Baird TD 

PROVIDER: S-EPMC5896957 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

ICE1 promotes the link between splicing and nonsense-mediated mRNA decay.

Baird Thomas D TD   Cheng Ken Chih-Chien KC   Chen Yu-Chi YC   Buehler Eugen E   Martin Scott E SE   Inglese James J   Hogg J Robert JR  

eLife 20180312


The nonsense-mediated mRNA decay (NMD) pathway detects aberrant transcripts containing premature termination codons (PTCs) and regulates expression of 5-10% of non-aberrant human mRNAs. To date, most proteins involved in NMD have been identified by genetic screens in model organisms; however, the increased complexity of gene expression regulation in human cells suggests that additional proteins may participate in the human NMD pathway. To identify proteins required for NMD, we performed a genome  ...[more]

Similar Datasets

2018-03-19 | GSE105436 | GEO
| PRJNA415177 | ENA
| S-EPMC7305299 | biostudies-literature
| S-EPMC5999336 | biostudies-literature
| S-EPMC1270949 | biostudies-literature
| S-EPMC2779665 | biostudies-literature
| S-EPMC3446288 | biostudies-literature
| S-EPMC6547761 | biostudies-literature
| S-EPMC2447145 | biostudies-literature
| S-EPMC4126864 | biostudies-literature