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Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage.

ABSTRACT: Fructophily is a rare trait that consists of the preference for fructose over other carbon sources. Here, we show that in a yeast lineage (the Wickerhamiella/Starmerella, W/S clade) comprised of fructophilic species thriving in the high-sugar floral niche, the acquisition of fructophily is concurrent with a wider remodeling of central carbon metabolism. Coupling comparative genomics with biochemical and genetic approaches, we gathered ample evidence for the loss of alcoholic fermentation in an ancestor of the W/S clade and subsequent reinstatement through either horizontal acquisition of homologous bacterial genes or modification of a pre-existing yeast gene. An enzyme required for sucrose assimilation was also acquired from bacteria, suggesting that the genetic novelties identified in the W/S clade may be related to adaptation to the high-sugar environment. This work shows how even central carbon metabolism can be remodeled by a surge of HGT events.

SUBMITTER: Goncalves C

PROVIDER: S-EPMC5897096 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage.

Gonçalves Carla C Wisecaver Jennifer H JH Kominek Jacek J Oom Madalena Salema MS Leandro Maria José MJ Shen Xing-Xing XX Opulente Dana A DA Zhou Xiaofan X Peris David D Kurtzman Cletus P CP Hittinger Chris Todd CT Rokas Antonis A Gonçalves Paula P

eLife 20180412

Fructophily is a rare trait that consists of the preference for fructose over other carbon sources. Here, we show that in a yeast lineage (the <i>Wickerhamiella</i>/<i>Starmerella</i>, W/S clade) comprised of fructophilic species thriving in the high-sugar floral niche, the acquisition of fructophily is concurrent with a wider remodeling of central carbon metabolism. Coupling comparative genomics with biochemical and genetic approaches, we gathered ample evidence for the loss of alcoholic fermen ...[more]

PMID: 29648535

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Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage.

Publications

Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage.

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