Ontology highlight
ABSTRACT:
SUBMITTER: Zheng G
PROVIDER: S-EPMC5897099 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
eLife 20180403
DNA replication transforms cohesin rings dynamically associated with chromatin into the cohesive form to establish sister-chromatid cohesion. Here, we show that, in human cells, cohesin loading onto chromosomes during early S phase requires the replicative helicase MCM2-7 and the kinase DDK. Cohesin and its loader SCC2/4 (NIPBL/MAU2 in humans) associate with DDK and phosphorylated MCM2-7. This binding does not require MCM2-7 activation by CDC45 and GINS, but its persistence on activated MCM2-7 r ...[more]