Impact of sequencing depth on the characterization of the microbiome and resistome.
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ABSTRACT:
SUBMITTER: Zaheer R
PROVIDER: S-EPMC5897366 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Publications
Impact of sequencing depth on the characterization of the microbiome and resistome.
Zaheer Rahat R Noyes Noelle N Ortega Polo Rodrigo R Cook Shaun R SR Marinier Eric E Van Domselaar Gary G Belk Keith E KE Morley Paul S PS McAllister Tim A TA
Scientific reports 20180412 1
Developments in high-throughput next generation sequencing (NGS) technology have rapidly advanced the understanding of overall microbial ecology as well as occurrence and diversity of specific genes within diverse environments. In the present study, we compared the ability of varying sequencing depths to generate meaningful information about the taxonomic structure and prevalence of antimicrobial resistance genes (ARGs) in the bovine fecal microbial community. Metagenomic sequencing was conducte ...[more]
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