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Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy.

ABSTRACT: Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave-optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.

SUBMITTER: Weigert M

PROVIDER: S-EPMC5898703 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Biobeam-Multiplexed wave-optical simulations of light-sheet microscopy.

Weigert Martin M Subramanian Kaushikaram K Bundschuh Sebastian T ST Myers Eugene W EW Kreysing Moritz M

PLoS computational biology 20180413 4

Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables simulation of operational light-sheet microscopes by combining data from 105-106 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive opti ...[more]

PMID: 29652879

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