Dataset Information

Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease.

ABSTRACT: Epidemiological studies have found a higher prevalence of allergic symptoms and positive prick tests in patients with Meniere's disease (MD); however the effect of allergenic extracts in MD has not been established. Thus, this study aims to determine the effect of Aspergillus and Penicillium stimulation in cytokine release and gene expression profile in MD. Patients with MD showed higher basal levels of IL-1?, IL-1RA, IL-6 and TNF-? when compared to healthy controls. We observed that IL-1? levels had a bimodal distribution suggesting two different subgroups of patients, with low and high basal levels of cytokines. Gene expression profile in peripheral blood mononuclear cells (PBMC) showed significant differences in patients with high and low basal levels of IL-1?. We found that both mold extracts triggered a significant release of TNF-? in MD patients, which were not found in controls. Moreover, after mold stimulation, MD patients showed a different gene expression profile in PBMC, according to the basal levels of IL-1?. The results indicate that a subset of MD patients have higher basal levels of proinflammatory cytokines and the exposure to Aspergillus and Penicillium extracts may trigger additional TNF-? release and contribute to exacerbate inflammation.

SUBMITTER: Frejo L

PROVIDER: S-EPMC5899176 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease.

Frejo Lidia L Gallego-Martinez Alvaro A Requena Teresa T Martin-Sanz Eduardo E Amor-Dorado Juan Carlos JC Soto-Varela Andres A Santos-Perez Sofia S Espinosa-Sanchez Juan Manuel JM Batuecas-Caletrio Angel A Aran Ismael I Fraile Jesus J Rossi-Izquierdo Marcos M Lopez-Escamez Jose Antonio JA

Scientific reports 20180413 1

Epidemiological studies have found a higher prevalence of allergic symptoms and positive prick tests in patients with Meniere's disease (MD); however the effect of allergenic extracts in MD has not been established. Thus, this study aims to determine the effect of Aspergillus and Penicillium stimulation in cytokine release and gene expression profile in MD. Patients with MD showed higher basal levels of IL-1β, IL-1RA, IL-6 and TNF-α when compared to healthy controls. We observed that IL-1β level ...[more]

PMID: 29654306

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Project description:Background and aimsObesity is a condition associated with chronic or acute inflammatory response characterized by an increase of proinflammatory cytokine levels. Peripheral blood mononuclear cells (PBMCs) migrate in adipose tissue inducing synthesis and secretion of adipocytokines as IL-6 and TNF-?. The aim of this study was to investigate the effect of berberine (a natural alkaloid) and red yeast (a natural antioxidant) on IL-6 and TNF-? cytokines release and gene expression, in circulating lipopolisaccarides (LPS) stimulated PBMCs.Methods and resultsPBMCs isolated from whole blood of healthy donors were stimulated with LPS to induce cytokines production; simultaneously cells were treated with increasing doses of berberine and red yeast. The substances were administered alone or in association. IL-6 and TNF-? protein levels in the culture medium and their mRNA levels were assessed by ELISA and real time PCR, respectively. Berberine and red yeast treatment prevented the LPS induction of IL-6 release in the culture medium of PBMCs. In addition, berberine plus red yeast treatment showed a synergic inhibitory effect on IL-6 release at low concentration. Berberine and red yeast showed an inhibitory effect also on LPS induction of TNF-? release exerting a synergic effect mainly at high concentrations. On the contrary, berberine and red yeast did not significantly affect IL-6 and TNF-? mRNA levels induced by LPS. In this case, only concomitant treatment of PBMCs with high doses of berberine and red yeast inhibits LPS induced IL-6 or TNF-? mRNA levels.ConclusionsThe results of our study show that both berberine and red yeast were able to carry out anti-inflammatory action through an inhibition of proinflammatory IL-6 and TNF-? protein release. Moreover, when given in combination these substances were able to inhibit IL-6 and TNF-? gene expression in PBMCs activated by LPS. Therefore, these substances could represent a useful pharmacological treatment to reduce the proinflammatory status accompanied with obesity.

Dataset Information

Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease.

Publications

Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease.

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