Dataset Information

Molecular markers associated with aluminium tolerance in Sorghum bicolor.

ABSTRACT: Sorghum (Sorghum bicolor, L. Moench) production in many agro-ecologies is constrained by a variety of stresses, including high levels of aluminium (Al) commonly found in acid soils. Therefore, for such soils, growing Al tolerant cultivars is imperative for high productivity.In this study, molecular markers associated with Al tolerance were identified using a mapping population developed by crossing two contrasting genotypes for this trait.Four SSR (Xtxp34, Sb5_236, Sb6_34, and Sb6_342), one STS (CTG29_3b) and three ISSR (811_1400, 835_200 and 884_200) markers produced alleles that showed significant association with Al tolerance. CTG29_3b, 811_1400, Xtxp34 and Sb5_236 are located on chromosome 3 with the first two markers located close to AltSB , a locus that underlie the Al tolerance gene (SbMATE) implying that their association with Al tolerance is due to their linkage to this gene. Although CTG29_3b and 811_1400 are located closer to AltSB , Xtxp34 and Sb5_236 explained higher phenotypic variance of Al tolerance indices. Markers 835_200, 884_200, Sb6_34 and Sb6_342 are located on different chromosomes, which implies the presence of several genes involved in Al tolerance in addition to SbMATE in sorghum.These molecular markers have a high potential for use in breeding for Al tolerance in sorghum.

SUBMITTER: Too EJ

PROVIDER: S-EPMC5899335 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Molecular markers associated with aluminium tolerance in <i>Sorghum bicolor</i>.

Too Emily Jepkosgei EJ Onkware Augustino Osoro AO Were Beatrice Ang'iyo BA Gudu Samuel S Carlsson Anders A Geleta Mulatu M

Hereditas 20180413

<h4>Background</h4>Sorghum (<i>Sorghum bicolor</i>, L. Moench) production in many agro-ecologies is constrained by a variety of stresses, including high levels of aluminium (Al) commonly found in acid soils. Therefore, for such soils, growing Al tolerant cultivars is imperative for high productivity.<h4>Methods</h4>In this study, molecular markers associated with Al tolerance were identified using a mapping population developed by crossing two contrasting genotypes for this trait.<h4>Results</h4 ...[more]

PMID: 29686601

Similar Datasets

Project description:BackgroundThe sequential nature of gel-based marker systems entails low throughput and high costs per assay. Commonly used marker systems such as SSR and SNP are also dependent on sequence information. These limitations result in high cost per data point and significantly limit the capacity of breeding programs to obtain sufficient return on investment to justify the routine use of marker-assisted breeding for many traits and particularly quantitative traits. Diversity Arrays Technology (DArT) is a cost effective hybridisation-based marker technology that offers a high multiplexing level while being independent of sequence information. This technology offers sorghum breeding programs an alternative approach to whole-genome profiling. We report on the development, application, mapping and utility of DArT markers for sorghum germplasm.ResultsA genotyping array was developed representing approximately 12,000 genomic clones using PstI+BanII complexity with a subset of clones obtained through the suppression subtractive hybridisation (SSH) method. The genotyping array was used to analyse a diverse set of sorghum genotypes and screening a Recombinant Inbred Lines (RIL) mapping population. Over 500 markers detected variation among 90 accessions used in a diversity analysis. Cluster analysis discriminated well between all 90 genotypes. To confirm that the sorghum DArT markers behave in a Mendelian manner, we constructed a genetic linkage map for a cross between R931945-2-2 and IS 8525 integrating DArT and other marker types. In total, 596 markers could be placed on the integrated linkage map, which spanned 1431.6 cM. The genetic linkage map had an average marker density of 1/2.39 cM, with an average DArT marker density of 1/3.9 cM.ConclusionWe have successfully developed DArT markers for Sorghum bicolor and have demonstrated that DArT provides high quality markers that can be used for diversity analyses and to construct medium-density genetic linkage maps. The high number of DArT markers generated in a single assay not only provides a precise estimate of genetic relationships among genotypes, but also their even distribution over the genome offers real advantages for a range of molecular breeding and genomics applications.

Dataset Information

Molecular markers associated with aluminium tolerance in Sorghum bicolor.

Publications

Molecular markers associated with aluminium tolerance in <i>Sorghum bicolor</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets