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Dysregulation of NEUROG2 plays a key role in focal cortical dysplasia.


ABSTRACT: OBJECTIVE:Focal cortical dysplasias (FCDs) are an important cause of drug-resistant epilepsy. In this work, we aimed to investigate whether abnormal gene regulation, mediated by microRNA, could be involved in FCD type II. METHODS:We used total RNA from the brain tissue of 16 patients with FCD type II and 28 controls. MicroRNA expression was initially assessed by microarray. Quantitative polymerase chain reaction, in situ hybridization, luciferase reporter assays, and deep sequencing for genes in the mTOR pathway were performed to validate and further explore our initial study. RESULTS:hsa-let-7f (p?=?0.039), hsa-miR-31 (p?=?0.0078), and hsa-miR34a (p?=?0.021) were downregulated in FCD type II, whereas a transcription factor involved in neuronal and glial fate specification, NEUROG2 (p?

SUBMITTER: Avansini SH 

PROVIDER: S-EPMC5901021 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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<h4>Objective</h4>Focal cortical dysplasias (FCDs) are an important cause of drug-resistant epilepsy. In this work, we aimed to investigate whether abnormal gene regulation, mediated by microRNA, could be involved in FCD type II.<h4>Methods</h4>We used total RNA from the brain tissue of 16 patients with FCD type II and 28 controls. MicroRNA expression was initially assessed by microarray. Quantitative polymerase chain reaction, in situ hybridization, luciferase reporter assays, and deep sequenci  ...[more]

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