Ontology highlight
ABSTRACT:
SUBMITTER: Born DA
PROVIDER: S-EPMC5901744 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Born David A DA Ulrich Emily C EC Ju Kou-San KS Peck Spencer C SC van der Donk Wilfred A WA Drennan Catherine L CL
Science (New York, N.Y.) 20171201 6368
Methylphosphonate synthase (MPnS) produces methylphosphonate, a metabolic precursor to methane in the upper ocean. Here, we determine a 2.35-angstrom resolution structure of MPnS and discover that it has an unusual 2-histidine-1-glutamine iron-coordinating triad. We further solve the structure of a related enzyme, hydroxyethylphosphonate dioxygenase from <i>Streptomyces albus</i> (<i>Sa</i>HEPD), and find that it displays the same motif. <i>Sa</i>HEPD can be converted into an MPnS by mutation of ...[more]