Ontology highlight
ABSTRACT:
SUBMITTER: Nibe Y
PROVIDER: S-EPMC5902196 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Nibe Yoichi Y Oshima Shigeru S Kobayashi Masanori M Maeyashiki Chiaki C Matsuzawa Yu Y Otsubo Kana K Matsuda Hiroki H Aonuma Emi E Nemoto Yasuhiro Y Nagaishi Takashi T Okamoto Ryuichi R Tsuchiya Kiichiro K Nakamura Tetsuya T Nakada Shinichiro S Watanabe Mamoru M
Autophagy 20180124 2
Ubiquitin chains are formed with 8 structurally and functionally distinct polymers. However, the functions of each polyubiquitin remain poorly understood. We developed a polyubiquitin-mediated fluorescence complementation (PolyUb-FC) assay using Kusabira Green (KG) as a split fluorescent protein. The PolyUb-FC assay has the advantage that monoubiquitination is nonfluorescent and chain-specific polyubiquitination can be directly visualized in living cells without using antibodies. We applied the ...[more]