Unknown

Dataset Information

0

Boxb mediate BALB/c mice corneal inflammation through a TLR4/MyD88-dependent signaling pathway in Aspergillus fumigatus keratitis.


ABSTRACT: AIM:To investigate whether high-mobility group box 1 (HMGB1) Boxb exacerbates BALB/c mice corneal immune responses and inflammatory through the Toll-like receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)-dependent signaling pathway in Aspergillus fumigatus (A. fumigatus) keratitis. METHODS:The mice corneas were pretreated with phosphate buffer saline (PBS), Boxb before A. fumigatus infection. The abdominal cavity extracted macrophages were pretreated with PBS, Boxb, TLR4 inhibitor (CLI-095), Dimethyl sulfoxide (DMSO) separately before A. fumigatus hyphae stimulation. HMGB1 was detected in normal and infected mice corneas and macrophages by real-time reverse transcriptase polymerase chain reaction (RT-PCR), the TLR4, MyD88, interleukin-1? (IL-1?), tumor necrosis factor-? (TNF-?) were detected by Western blot and PCR. RESULTS:In BALB/c mice corneas, the expressions of TLR4, HMGB1, IL-1?, TNF-? were increased after A. fumigatus infection. While pretreatment with Boxb significantly increased the expressions of TLR4, HMGB1, MyD88, IL-1?, TNF-? compared with PBS control after infection. In BALB/c mice abdominal cavity extracted macrophages, pretreatment with Boxb increased the expressions of TLR4, HMGB1, MyD88, IL-1?, TNF-?, while pretreatment with CLI-095 and Boxb significantly decreased the expressions of TLR4, HMGB1, MyD88, IL-1?, TNF-?. CONCLUSION:In A. fumigatus keratitis, Boxb play a pro-inflammatory role in corneal anti-fungi immune response through the HMGB1-TLR4-MyD88 signal pathway.

SUBMITTER: Liu M 

PROVIDER: S-EPMC5902355 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

altmetric image

Publications

Boxb mediate BALB/c mice corneal inflammation through a TLR4/MyD88-dependent signaling pathway in <i>Aspergillus fumigatus</i> keratitis.

Liu Min M   Li Cui C   Zhao Gui-Qiu GQ   Lin Jing J   Che Cheng-Ye CY   Xu Qiang Q   Wang Qian Q   Xu Rui R   Niu Ya-Wen YW  

International journal of ophthalmology 20180418 4


<h4>Aim</h4>To investigate whether high-mobility group box 1 (HMGB1) Boxb exacerbates BALB/c mice corneal immune responses and inflammatory through the Toll-like receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)-dependent signaling pathway in <i>Aspergillus fumigatus</i> (<i>A. fumigatus</i>) keratitis.<h4>Methods</h4>The mice corneas were pretreated with phosphate buffer saline (PBS), Boxb before <i>A. fumigatus</i> infection. The abdominal cavity extracted macrophages were  ...[more]

Similar Datasets

| S-EPMC2895653 | biostudies-literature
| S-EPMC7013794 | biostudies-literature
| S-EPMC7415897 | biostudies-literature
| S-EPMC6520258 | biostudies-literature
| S-EPMC8267217 | biostudies-literature
| S-EPMC4567309 | biostudies-literature
| S-EPMC6393676 | biostudies-other
| S-EPMC5221624 | biostudies-literature
| S-EPMC6520267 | biostudies-literature
| S-EPMC7425693 | biostudies-literature