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Extended depth of focus for coherence-based cellular imaging.

ABSTRACT: Improving lateral resolution for cross-sectional optical coherence tomography (OCT) imaging is difficult due to the rapid divergence of light once it is focused to a small spot. To overcome this obstacle, we introduce a fiber optics system that generates a coaxially focused multimode (CAFM) beam for depth of focus (DOF) extension. We fabricated a CAFM beam OCT probe and show that the DOF is more than fivefold that of a conventional Gaussian beam, enabling cross-sectional imaging of biological tissues with clearly resolved cellular and subcellular structures over more than a 400 ?m depth range. The compact and straightforward design and high-resolution extended DOF imaging capabilities of this technique suggests that it will be very useful for endoscopic cross-sectional imaging of human internal organs in vivo.

SUBMITTER: Yin B

PROVIDER: S-EPMC5902383 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Extended depth of focus for coherence-based cellular imaging.

Yin Biwei B Hyun Chulho C Gardecki Joseph A JA Tearney Guillermo J GJ

Optica 20170809 8

Improving lateral resolution for cross-sectional optical coherence tomography (OCT) imaging is difficult due to the rapid divergence of light once it is focused to a small spot. To overcome this obstacle, we introduce a fiber optics system that generates a coaxially focused multimode (CAFM) beam for depth of focus (DOF) extension. We fabricated a CAFM beam OCT probe and show that the DOF is more than fivefold that of a conventional Gaussian beam, enabling cross-sectional imaging of biological ti ...[more]

PMID: 29675447

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Visual performance and patient preference with bilateral implantation of an extended depth of focus or combined implantation of an extended depth of focus/trifocal intraocular lens

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