Dataset Information

Surface micropattern reduces colonization and medical device-associated infections.

ABSTRACT:

Purpose

Surface microtopography offers a promising approach for infection control. The goal of this study was to provide evidence that micropatterned surfaces significantly reduce the potential risk of medical device-associated infections.

Methodology

Micropatterned and smooth surfaces were challenged in vitro against the colonization and transference of two representative bacterial pathogens - Staphylococcus aureus and Pseudomonas aeruginosa. A percutaneous rat model was used to assess the effectiveness of the micropattern against device-associated S. aureus infections. After the percutaneous insertion of silicone rods into (healthy or immunocompromised) rats, their backs were inoculated with S. aureus. The bacterial burdens were determined in tissues under the rods and in the spleens.

Results

The micropatterns reduced adherence by S. aureus (92.3 and 90.5?%?reduction for flat and cylindrical surfaces, respectively), while P. aeruginosa colonization was limited by 99.9?% (flat) and 95.5?% (cylindrical). The micropatterned surfaces restricted transference by 95.1?% for S. aureus and 94.9?% for P. aeruginosa, compared to smooth surfaces. Rats with micropatterned devices had substantially fewer S. aureus in subcutaneous tissues (91?%) and spleens (88?%) compared to those with smooth ones. In a follow-up study, immunocompromised rats with micropatterned devices had significantly lower bacterial burdens on devices (99.5 and 99.9?%?reduction on external and internal segments, respectively), as well as in subcutaneous tissues (97.8?%) and spleens (90.7?%) compared to those with smooth devices.

Conclusion

Micropatterned surfaces exhibited significantly reduced colonization and transference in vitro, as well as lower bacterial burdens in animal models. These results indicate that introducing this micropattern onto surfaces has high potential to reduce medical device-associated infections.

SUBMITTER: Xu B

PROVIDER: S-EPMC5903250 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Publications

Surface micropattern reduces colonization and medical device-associated infections.

Xu Binjie B Wei Qiuhua Q Mettetal M Ryan MR Han Jie J Rau Lindsey L Tie Jinfeng J May Rhea M RM Pathe Eric T ET Reddy Shravanthi T ST Sullivan Lauren L Parker Albert E AE Maul Donald H DH Brennan Anthony B AB Mann Ethan E EE

Journal of medical microbiology 20171006 11

<h4>Purpose</h4>Surface microtopography offers a promising approach for infection control. The goal of this study was to provide evidence that micropatterned surfaces significantly reduce the potential risk of medical device-associated infections.<h4>Methodology</h4>Micropatterned and smooth surfaces were challenged in vitro against the colonization and transference of two representative bacterial pathogens - Staphylococcus aureus and Pseudomonas aeruginosa. A percutaneous rat model was used to ...[more]

PMID: 28984233

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Project description:BackgroundLeft ventricular assist device (LVAD)-associated infections are major complications that can lead to critical outcomes. The aims of this study were to assess the incidence of and to determine the risk factors for LVAD-associated infections.MethodsWe included all consecutive patients undergoing LVAD implantation between January 1, 2010, and January 1, 2019, in a single institution. Infection-related data were retrospectively collected by review of patient’s medical files. LVAD-associated infections were classified into three categories: percutaneous driveline infections, pocket infections and pump and/or cannula infections.ResultsWe enrolled 72 patients. Twenty-one (29.2%) patients presented a total of 32 LVAD-associated infections. Eight (38.1%) patients had more than one infection. Five (62.5%) pocket infections and one (50.0%) pump and/or cannula infection were preceded by a driveline infection. The median delay between the operation and LVAD-associated infection was 6.5 (1.4–12.4) months. The probability of having a LVAD-associated infection at one year after receiving an implant was 26.6% (95% CI: 17.5–40.5%). Percutaneous driveline infections represented 68.7% of all LVAD-associated infections. Staphylococcus aureus and coagulase-negative staphylococci were the predominant bacteria in LVAD-associated infections (53.1% and 15.6%, respectively). Hospital length of stay (sdHR =1.22 per 10 days; P=0.001) and postoperative hemodialysis (sdHR =0.17; P=0.004) were statistically associated with infection. Colonization with multidrug-resistant bacteria was more frequent in patients with LVAD-associated infections than in others patients (42.9% vs. 15.7%; P=0.013).ConclusionsLVAD-associated infections remain an important complication and are mostly represented by percutaneous driveline infections. Gram-positive cocci are the main pathogens isolated in microbiological samples. Patients with LVAD-associated infections are more frequently colonized with multidrug-resistant bacteria.

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Dataset Information

Surface micropattern reduces colonization and medical device-associated infections.

Purpose

Methodology

Results

Conclusion

Publications

Surface micropattern reduces colonization and medical device-associated infections.

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