Dataset Information

Association of NLRP1 and NLRP3 Polymorphisms with Psoriasis Vulgaris Risk in the Chinese Han Population.

ABSTRACT: Aim:To clarify the association between the single nucleotide polymorphisms (SNPs) in the NLRP1 and NLRP3 and Psoriasis Vulgaris (PsV) in the Chinese Han population. Methods:We genotyped eight SNPs, four from NLRP1 (rs8079034, rs11651270, rs11657747, and rs878329) and NLRP3 (rs7512998, rs3806265, rs10754557, and rs10733113) each in 540 patients with PsV and 612 healthy controls in the Chinese Han population using an improved multiplexed ligation detection reaction (iMLDR) method. The genotype and haplotype frequencies were analyzed using a case-control study design. Results:We identified two SNPs, rs3806265 and rs10754557, in NLRP3 that were significantly associated with PsV. The genotype distribution of the rs3806265 SNP was significantly different between cases and controls (p = 0.0451; OR = 0.791; 95% CI = 0.627-0.998). In the recessive model, the genotype distribution of the rs10754557 SNP was significantly different between cases and controls (p = 0.0344; OR = 1.277; 95% CI = 0.987-1.652). The haplotype analysis of rs3806265 and rs10754557 also presented a significant association of TA haplotype with PsV (?2 = 4.529; p = 0.033). Conclusion:NLRP3 may play a role in PsV susceptibility in the Chinese Han population.

SUBMITTER: Yu P

PROVIDER: S-EPMC5903344 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Association of <i>NLRP1</i> and <i>NLRP3</i> Polymorphisms with Psoriasis Vulgaris Risk in the Chinese Han Population.

Yu Pei P Hao Siyu S Zheng Hewei H Zhao Xueying X Li Yuzhen Y

BioMed research international 20180403

<h4>Aim</h4>To clarify the association between the single nucleotide polymorphisms (SNPs) in the <i>NLRP1</i> and <i>NLRP3</i> and Psoriasis Vulgaris (PsV) in the Chinese Han population.<h4>Methods</h4>We genotyped eight SNPs, four from <i>NLRP1</i> (rs8079034, rs11651270, rs11657747, and rs878329) and <i>NLRP3</i> (rs7512998, rs3806265, rs10754557, and rs10733113) each in 540 patients with PsV and 612 healthy controls in the Chinese Han population using an improved multiplexed ligation detectio ...[more]

PMID: 29850521

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Project description:Psoriasis is a chronic inflammatory skin disease related to immune, whose complexity of molecular mechanisms is still not fully clear. RNA sequencing has been widely applied in various fields including biological medicine. According to the bioinformatics analysis of differential genes, biomarkers and drug targets have been discovered for the diagnosis and treatment of diseases. Besides, the pathological mechanisms of disease and functions of gene can be evaluated. In the present study, we report the application of RNA sequencing in skin tissues from psoriatic and healthy persons. By obtaining 2139 differential expressed genes (DEGs), 208 significantly differential GO terms and 44 significantly differential pathways were generated. We found that the functions of DEGs were mainly related to cell cycle, inflammatory, virus, immune response and metabolic process.The major pathways included cytokine-cytokine receptor interaction, Toll-like receptor signaling pathway, chemokine signaling pathway, cell cycle, metabolic pathways, ribosome, peroxisome, steroid biosynthesis and biosynthesis of unsaturated fatty acids. Furthermore, co-expression network was constructed to identify core genes and relations between genes. we considered genes with high values of degree and k-core difference in the co-expression network as core genes, such as IFNG, IL26, TLR3, PRKCQ, TLR4, CD274, CDK1 and IL17A. We chose CD274, an important immune checkpoint, to evaluate its regulatory mechanisms. Candidate genes related to CD274 were evaluated by the co-expression network analysis, and the relations between CD274 and candidate genes were validated in epidermal keratinocytes. Finally, IFNG and CDK1 inhibitor (indirubin) were found increasing the expression levels of CD274. In addition, indirubin was confirmed to attenuate mouse psoriasis-like skin lesion with the mechanisms related to CD274. In conclusion, this study provides us a comprehensive transcriptome analysis method on psoriasis to identify core genes and explore the important regulatory functions of genes.

Dataset Information

Association of NLRP1 and NLRP3 Polymorphisms with Psoriasis Vulgaris Risk in the Chinese Han Population.

Publications

Association of <i>NLRP1</i> and <i>NLRP3</i> Polymorphisms with Psoriasis Vulgaris Risk in the Chinese Han Population.

Similar Datasets

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