Ontology highlight
ABSTRACT:
SUBMITTER: Shirian J
PROVIDER: S-EPMC5903968 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
FEBS letters 20180312 7
MMP-14 and MMP-9 are two well-established cancer targets for which no specific clinically relevant inhibitor is available. Using a powerful combination of computational design and yeast surface display technology, we engineered such an inhibitor starting from a nonspecific MMP inhibitor, N-TIMP2. The engineered purified N-TIMP2 variants showed enhanced specificity toward MMP-14 and MMP-9 relative to a panel of off-target MMPs. MMP-specific N-TIMP2 sequence signatures were obtained that could be ...[more]