Ontology highlight
ABSTRACT:
SUBMITTER: Tso SC
PROVIDER: S-EPMC5906060 | biostudies-literature | 2018 Jan
REPOSITORIES: biostudies-literature
Tso Shih-Chia SC Chen Qiuyan Q Vishnivetskiy Sergey A SA Gurevich Vsevolod V VV Iverson T M TM Brautigam Chad A CA
Analytical biochemistry 20171018
The emergence of microscale thermophoresis (MST) as a technique for determining the dissociation constants for bimolecular interactions has enabled these quantities to be measured in systems that were previously difficult or impracticable. However, most models for analyses of these data featured the assumption of a simple 1:1 binding interaction. The only model widely used for multiple binding sites was the Hill equation. Here, we describe two new MST analytic models that assume a 1:2 binding sc ...[more]