Ontology highlight
ABSTRACT:
SUBMITTER: Morita D
PROVIDER: S-EPMC5907825 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Morita Daisuke D Nishio Nobuhiro N Saito Shoji S Tanaka Miyuki M Kawashima Nozomu N Okuno Yusuke Y Suzuki Satoshi S Matsuda Kazuyuki K Maeda Yasuhiro Y Wilson Matthew H MH Dotti Gianpietro G Rooney Cliona M CM Takahashi Yoshiyuki Y Nakazawa Yozo Y
Molecular therapy. Methods & clinical development 20171222
Adoptive T cell therapy using chimeric antigen receptor (CAR)-modified T cells is a promising cancer immunotherapy. We previously developed a non-viral method of gene transfer into T cells using a <i>piggyBac</i> transposon system to improve the cost-effectiveness of CAR-T cell therapy. Here, we have further improved our technology by a novel culture strategy to increase the transfection efficiency and to reduce the time of T cell manufacturing. Using a CH2CH3-free CD19-specific CAR transposon v ...[more]