Measurement of plasma norepinephrine and 3,4-dihydroxyphenylglycol: method development for a translational research study.

ABSTRACT:

Objective

Norepinephrine (NE), a sympathetic neurotransmitter, is often measured in plasma as an index of sympathetic activity. To better understand NE dynamics, it is important to measure its principal metabolite, 3,4-dihydroxyphenylglycol (DHPG), concurrently. Our aim was to present a method, developed in the course of a translational research study, to measure NE and DHPG in human plasma using high performance liquid chromatography with electrochemical detection (HPLC-ED).

Results

After pre-purifying plasma samples by alumina extraction, we used HPLC-ED to separate and quantify NE and DHPG. In order to remove uric acid, which co-eluted with DHPG, a sodium bicarbonate wash was added to the alumina extraction procedure, and we oxidized the column eluates followed by reducti

SUBMITTER: Denfeld QE 

PROVIDER: S-EPMC5909231 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature