Modeling the dynamics and kinetics of HIV-1 Gag during viral assembly.

ABSTRACT: We report a computational model for the assembly of HIV-1 Gag into immature viral particles at the plasma membrane. To reproduce experimental structural and kinetic properties of assembly, a process occurring on the order of minutes, a coarse-grained representation consisting of a single particle per Gag molecule is developed. The model uses information relating the functional interfaces implicated in Gag assembly, results from cryo electron-tomography, and biophysical measurements from fluorescence microscopy, such as the dynamics of Gag assembly at single virions. These experimental constraints eliminated many classes of potential interactions, and narrowed the model to a single interaction scheme with two non-equivalent interfaces acting to form Gags into a hexamer, and a third interfac

SUBMITTER: Tomasini MD 

PROVIDER: S-EPMC5909904 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature