Ontology highlight
ABSTRACT:
SUBMITTER: Lyu Z
PROVIDER: S-EPMC5910183 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Lyu Zhigang Z Kang Lei L Buuh Zakey Yusuf ZY Jiang Dawei D McGuth Jeffrey C JC Du Juanjuan J Wissler Haley L HL Cai Weibo W Wang Rongsheng E RE
ACS chemical biology 20180228 4
Genetic incorporation of unnatural amino acids (UAAs) provides a unique approach to the synthesis of site-specific antibody conjugates that are homogeneous and better defined constructs than random conjugates. Yet, the yield varies for every antibody, and the process is costly and time-consuming. We have developed a switchable αGCN4-Fab conjugate that incorporates UAA p-acetylphenylalanine. The GCN4 peptide is used as a switch, and antibodies fused by GCN4 can direct the αGCN4-Fab conjugate to t ...[more]