A Stress Response that Monitors and Regulates mRNA Structure Is Central to Cold Shock Adaptation.

ABSTRACT: Temperature influences the structural and functional properties of cellular components, necessitating stress responses to restore homeostasis following temperature shift. Whereas the circuitry controlling the heat shock response is well understood, that controlling the E. coli cold shock adaptation program is not. We found that during the growth arrest phase (acclimation) that follows shift to low temperature, protein synthesis increases, and open reading frame (ORF)-wide mRNA secondary structure decreases. To identify the regulatory system controlling this process, we screened for players required for increased translation. We identified a two-member mRNA surveillance system that enables recovery of translation during acclimation: RNase R assures appropriate mRNA degradation and the Csps

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC5910227 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature