Dataset Information

CD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis.

ABSTRACT: Mycobacterial cell wall lipids bind the conserved CD1 family of antigen-presenting molecules and activate T cells via their T cell receptors (TCRs). Sulfoglycolipids (SGLs) are uniquely synthesized by Mycobacterium tuberculosis, but tools to study SGL-specific T cells in humans are lacking. We designed a novel hybrid synthesis of a naturally occurring SGL, generated CD1b tetramers loaded with natural or synthetic SGL analogs, and studied the molecular requirements for TCR binding and T cell activation. Two T cell lines derived using natural SGLs are activated by synthetic analogs independently of lipid chain length and hydroxylation, but differentially by saturation status. By contrast, two T cell lines derived using an unsaturated SGL synthetic analog were not activated by the natural antigen. Our data provide a bioequivalence hierarchy of synthetic SGL analogs and SGL-loaded CD1b tetramers. These reagents can now be applied to large-scale translational studies investigating the diagnostic potential of SGL-specific T cell responses or SGL-based vaccines.

SUBMITTER: James CA

PROVIDER: S-EPMC5910231 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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CD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis.

James Charlotte A CA Yu Krystle K Q KKQ Gilleron Martine M Prandi Jacques J Yedulla Vijayendar R VR Moleda Zuzanna Z ZZ Diamanti Eleonora E Khan Momin M Aggarwal Varinder K VK Reijneveld Josephine F JF Reinink Peter P Lenz Stefanie S Emerson Ryan O RO Scriba Thomas J TJ Souter Michael N T MNT Godfrey Dale I DI Pellicci Daniel G DG Moody D Branch DB Minnaard Adriaan J AJ Seshadri Chetan C Van Rhijn Ildiko I

Cell chemical biology 20180201 4

Mycobacterial cell wall lipids bind the conserved CD1 family of antigen-presenting molecules and activate T cells via their T cell receptors (TCRs). Sulfoglycolipids (SGLs) are uniquely synthesized by Mycobacterium tuberculosis, but tools to study SGL-specific T cells in humans are lacking. We designed a novel hybrid synthesis of a naturally occurring SGL, generated CD1b tetramers loaded with natural or synthetic SGL analogs, and studied the molecular requirements for TCR binding and T cell acti ...[more]

PMID: 29398561

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Dataset Information

CD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis.

Publications

CD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis.

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