Ontology highlight
ABSTRACT:
SUBMITTER: Younger AKD
PROVIDER: S-EPMC5914420 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Younger Andrew K D AKD Su Peter Y PY Shepard Andrea J AJ Udani Shreya V SV Cybulski Thaddeus R TR Tyo Keith E J KEJ Leonard Joshua N JN
Protein engineering, design & selection : PEDS 20180201 2
Naturally evolved metabolite-responsive biosensors enable applications in metabolic engineering, ranging from screening large genetic libraries to dynamically regulating biosynthetic pathways. However, there are many metabolites for which a natural biosensor does not exist. To address this need, we developed a general method for converting metabolite-binding proteins into metabolite-responsive transcription factors-Biosensor Engineering by Random Domain Insertion (BERDI). This approach takes adv ...[more]