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Plug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry.

ABSTRACT: Systematic approaches to studying cellular signaling require phosphoproteomic techniques that reproducibly measure the same phosphopeptides across multiple replicates, conditions, and time points. Here we present a method to mine information from large-scale, heterogeneous phosphoproteomics data sets to rapidly generate robust targeted mass spectrometry (MS) assays. We demonstrate the performance of our method by interrogating the IGF-1/AKT signaling pathway, showing that even rarely observed phosphorylation events can be consistently detected and precisely quantified.

SUBMITTER: Lawrence RT

PROVIDER: S-EPMC5915315 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Plug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry.

Lawrence Robert T RT Searle Brian C BC Llovet Ariadna A Villén Judit J

Nature methods 20160328 5

Systematic approaches to studying cellular signaling require phosphoproteomic techniques that reproducibly measure the same phosphopeptides across multiple replicates, conditions, and time points. Here we present a method to mine information from large-scale, heterogeneous phosphoproteomics data sets to rapidly generate robust targeted mass spectrometry (MS) assays. We demonstrate the performance of our method by interrogating the IGF-1/AKT signaling pathway, showing that even rarely observed ph ...[more]

PMID: 27018578

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