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Dacarbazine depletes the ovarian reserve in mice and depletion is enhanced with age.

ABSTRACT: Dacarbazine is commonly administered for the treatment of cancers prevalent in reproductive age females. However, investigations of off-target effects of dacarbazine on the ovary are limited. We assessed the impact of dacarbazine on the ovarian reserve of primordial follicles, essential for fertility. Eight week and 6 month old C57BL/6?J mice were administered with dacarbazine or saline on day (d)0 and d7, then sacrificed after 12?hours (h), or 14d (n?=?4-5/group). Follicle numbers, follicle density, serum AMH and corpora lutea were quantified and estrous cyclicity monitored. In reproductively young mice, dacarbazine did not affect primordial follicle numbers at 12?h, but resulted in a 36% reduction at 14d (p?

SUBMITTER: Winship AL

PROVIDER: S-EPMC5917018 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Dacarbazine depletes the ovarian reserve in mice and depletion is enhanced with age.

Winship Amy L AL Bakai Monika M Sarma Urooza U Liew Seng H SH Hutt Karla J KJ

Scientific reports 20180425 1

Dacarbazine is commonly administered for the treatment of cancers prevalent in reproductive age females. However, investigations of off-target effects of dacarbazine on the ovary are limited. We assessed the impact of dacarbazine on the ovarian reserve of primordial follicles, essential for fertility. Eight week and 6 month old C57BL/6 J mice were administered with dacarbazine or saline on day (d)0 and d7, then sacrificed after 12 hours (h), or 14d (n = 4-5/group). Follicle numbers, follicle den ...[more]

PMID: 29695822

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