Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes.
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ABSTRACT:
SUBMITTER: Horvath A
PROVIDER: S-EPMC5918194 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Publications
Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes.
Horváth András A Lemoine Marc D MD Löser Alexandra A Mannhardt Ingra I Flenner Frederik F Uzun Ahmet Umur AU Neuber Christiane C Breckwoldt Kaja K Hansen Arne A Girdauskas Evaldas E Reichenspurner Hermann H Willems Stephan S Jost Norbert N Wettwer Erich E Eschenhagen Thomas T Christ Torsten T
Stem cell reports 20180208 3
Human induced pluripotent stem cell (hiPSC) cardiomyocytes (CMs) show less negative resting membrane potential (RMP), which is attributed to small inward rectifier currents (I<sub>K1</sub>). Here, I<sub>K1</sub> was measured in hiPSC-CMs (proprietary and commercial cell line) cultured as monolayer (ML) or 3D engineered heart tissue (EHT) and, for direct comparison, in CMs from human right atrial (RA) and left ventricular (LV) tissue. RMP was measured in isolated cells and intact tissues. I<sub>K ...[more]
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