Ontology highlight
ABSTRACT:
SUBMITTER: Geister KA
PROVIDER: S-EPMC5919724 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Geister Krista A KA Timms Andrew E AE Beier David R DR
G3 (Bethesda, Md.) 20180202 2
Positional cloning of ENU-induced mutations has traditionally relied on analysis of polymorphic variation between two strains. In contrast, the application of whole-genome sequencing (WGS) has enabled gene discovery in mutant lines maintained on an inbred genetic background. This approach utilizes genetic variation derived from ENU-induced variants for mapping and reduces the likelihood of phenotypic variation, making it an ideal method for genetic modifier screening. Here, we describe the resul ...[more]