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NOTCH1 Is Aberrantly Activated in Chronic Lymphocytic Leukemia Hematopoietic Stem Cells.

ABSTRACT: To investigate chronic lymphocytic leukemia (CLL)-initiating cells, we assessed NOTCH1 mutation/expression in hematopoietic stem cells (HSCs). In NOTCH1-mutated CLL, we detected subclonal mutations in 57% CD34+/CD38- HSCs. NOTCH1 mutation was present in 66% CD34+/CD38+ progenitor cells displaying an increased mutational burden compared to HSCs. Flow cytometric analysis revealed significantly higher NOTCH1 activation in CD34+/CD38- and CD34+/CD38+ cells from CLL patients, regardless NOTCH1 mutation compared to healthy donors. Activated NOTCH1 resulted in overexpression of the NOTCH1 target c-MYC. We conclude that activated NOTCH1 is an early event in CLL that may contribute to aberrant HSCs in this disease.

SUBMITTER: Di Ianni M 

PROVIDER: S-EPMC5919960 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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To investigate chronic lymphocytic leukemia (CLL)-initiating cells, we assessed <i>NOTCH1</i> mutation/expression in hematopoietic stem cells (HSCs). In <i>NOTCH1-</i>mutated CLL, we detected subclonal mutations in 57% CD34+/CD38- HSCs. <i>NOTCH1</i> mutation was present in 66% CD34+/CD38+ progenitor cells displaying an increased mutational burden compared to HSCs. Flow cytometric analysis revealed significantly higher NOTCH1 activation in CD34+/CD38- and CD34+/CD38+ cells from CLL patients, reg  ...[more]

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