Dataset Information

Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH.

ABSTRACT:

Background

A great interest exists in the production of hybrid plants of the genus Passiflora given the beauty and exotic features of its flowers which have ornamental value. Hybrid paternity confirmation is therefore important for assuring germplasm origin, and is typically carried out by molecular marker segregation. The aim of this study was to karyotypically characterize the chromosome heritance patterns of the progeny resultant from a cross of P. gardneri and P. gibertii using classical cytogenetics, chromosome banding, and molecular cytogenetics.

Results

All analyzed genotypes showed the same diploid chromosome number as the genitor species: 2n = 18. Classical and CMA₃ and DAPI staining allowed for chromosome counting and satellite identification (secondary constrictions). Fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) were used to characterize subgenomes by either identifying rDNA-specific genome patterns or parental genomes, respectively.

Conclusions

The heritance of chromosomal markers presenting rDNA sites from each parent for genome identification confirmed that all obtained plants were hybrids. These results will improve breeding programs involving the species of this genus. Apart from confirming hybridization, GISH allowed the visualization of recombination between the homeologous chromosome and the introgression of sequences of interest.

SUBMITTER: Silva GS

PROVIDER: S-EPMC5921547 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature

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Publications

Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH.

Silva Gonçalo Santos GS Souza Margarete Magalhães MM de Melo Cláusio Antônio Ferreira CAF Urdampilleta Juan Domingo JD Forni-Martins Eliana Regina ER

BMC genetics 20180427 1

<h4>Background</h4>A great interest exists in the production of hybrid plants of the genus Passiflora given the beauty and exotic features of its flowers which have ornamental value. Hybrid paternity confirmation is therefore important for assuring germplasm origin, and is typically carried out by molecular marker segregation. The aim of this study was to karyotypically characterize the chromosome heritance patterns of the progeny resultant from a cross of P. gardneri and P. gibertii using class ...[more]

PMID: 29703134

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Dataset Information

Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH.

Background

Results

Conclusions

Publications

Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH.

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